Knockdown of 60S ribosomal protein L14-2 reveals their potential regulatory roles to enhance drought and salt tolerance in cotton

Abstract Background Cotton is a valuable economic crop and the main significant source of natural fiber for textile industries globally. The effects drought salt stress pose challenge to strong large-scale production due ever-changing climatic conditions. However, plants have evolved number survival strategies, among them induction various stress-responsive genes such as ribosomal protein large ( RPL ) gene. gene families encode critical proteins, which alleviate in plants. In this study, comprehensive functional analysis cotton was carried out under stresses. Results Based on genome-wide evaluation, 26, 8, 5 proteins containing RPL14B domain were identified Gossypium hirsutum , G. raimondii arboreum respectively. Furthermore, through bioinformatics analysis, key cis -regulatory elements related discovered. Myb binding sites (MBS), abscisic acid-responsive element (ABRE), CAAT-box, TATA box, TGACG-motif, CGTCA-motif responsive methyl jasmonate, well TCA-motif salicylic acid, identified. Expression revealed gene, Gh_D01G0234 (RPL14B), with significantly higher levels further evaluated reverse genetic approach. knockdown (RPL14B) affected performance seedlings drought/salt conditions, evidenced by substantial reduction morphological physiological traits. Moreover, level antioxidant enzyme reduced VIGS-plants, while oxidant increased significantly, demonstrated malondialdehyde concentration level. Conclusion results potential role promoting enzymes, are oxidizing oxidants. pathways need be investigated even we exploit these developing more stress-resilient germplasms.